Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay
نویسندگان
چکیده
منابع مشابه
Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay
A review of using Griess Reaction method to determine nitric oxide production in biological systems.
متن کاملMeasurement of nitric oxide in biological models.
Nitric oxide (NO) is a small, gaseous, paramagnetic radical with a high affinity for interaction with ferrous hemoproteins such as soluble guanylate cyclase and hemoglobin. Interest in NO measurement increased exponentially with the discovery that NO or a related compound is the endothelium-derived relaxing factor (EDRF). In addition to being a potent endogenous vasodilator, NO has a role in in...
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The chemistry of peroxynitrite is complex and highly pHdependent [6,7]. Not only peroxynitrite anion (ONOO-) and its conjugated acid, peroxynitrous acid (ONOOH) (pKa = 6.8) are potent oxidants by themselves [8,9] but also they can lead to the production of secondary reactive species. Moreover, peroxynitrite is capable of nitrating aromatics [10] in a process that can be enhanced by metal centre...
متن کاملNitric oxide and superoxide in biological systems
DETC: diethyldithiocarbamate, DMPO: 5,5-dimethyl-1-pyrroline N-oxide, DNIC: di-nitrosyl-iron complex, DTCS: N-(dithiocarboxy)sarcosine, EDRF: endothelium-derived relaxing factor, EPR, ESR: electron paramagnetic resonance; electron spin resonance, cGMP: cyclic GMP, Hb: hemoglobin, LPS: lipopolysaccharide, Mb: myoglobin, MGD: N-methyl-D-glucamine dithiocarbamate, MNIC: mono-nitrosyl-iron complex,...
متن کاملNitric oxide and superoxide in biological systems
Oxygen-derived free radicals play a major role in the physiology and pathology of the vessel wall [1]. To assess superoxide anion production (O2) from endothelium, four techniques are mainly used: reduced cytochrome c UV-visible absorption [2,3], ESR spin trapping [4,5], hydroethydine fluorescence [6] and lucigenin-enhanced chemiluminescence [7,8]. The simplest technique which has been used for...
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ژورنال
عنوان ژورنال: Sensors
سال: 2003
ISSN: 1424-8220
DOI: 10.3390/s30800276